生物通报道 来自昆明动物所的消息，研究所动物模型与人类疾病机理重点实验室的研究人员在对云南省的1294名汉族或傣族静脉吸毒者的研究中取得了关于TRIM5α的基因多态性与HIV-1易感性的相关性的最新进展，研究成果近期发表在国际著名的艾滋病学术期刊JAIDS (56:306-311, 2011)上。
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领导这一研究的是昆明动物研究所郑永唐研究员，郑永唐研究员现为中国科学院昆明动物研究所分子免疫药理学实验室主任，1996 年首批入选中国科学院“西部之光”人才。 1997 年被云南省人民政府授予 “云南省中青年学术和技术学科带头人”称号。 2004 年获云南省有突出贡献的优秀专业技术人才。其他作者包括博士生刘丰亮和香港中文大学邓亮生教授。
在生物的整个进化过程中，生物体进化出了各种“武器”来对抗各种病毒的入侵。为了对付HIV-1的感染，灵长类针对HIV-1在细胞内的复制过程产生了各种抑制HIV-1病毒复制的限制因子，如三模体蛋白5α(TRIM5α)、载脂蛋白B mRNA编辑酶3G(APOBEC3G)、束缚蛋白(tetherin) 和锌指结构抗病毒蛋白(ZAP)等。限制因子的基因多态性往往与个体对病毒易感性和感染后病程进展的差异密切相关。人TRIM5α的基因多态性与HIV-1易感性及病程进展相关性研究所得到的结论并不一致，而且缺乏中国规模人群TRIM5α多态性的研究。
在这篇文章中，研究人员对云南省的1294名汉族或傣族静脉吸毒者的TRIM5α的基因多态性与HIV-1易感性的相关性进行研究。研究共发现14个基因变异，除之前已有报道的-2G/C、H43Y、V112F、R136Q等几个重要的SNP位点之外，还新发现了I2V、R97C、G110R等罕见的突变位点。对4个基因频率较高的SNP(即常见SNP)进行相关性分析，结果发现-2G/C、V112F和R136Q这3个SNP的基因型频率和基因频率以及4个常见SNP的单倍体在HIV-1阳性群体和阴性群体中的分布没有统计学差异，而H43Y的纯合突变基因型(TT)在静脉吸毒HIV-1感染者中频率显著低于静脉吸毒HIV-1未感染者，并且这种统计学差异在对数据进行民族方面的分层分析后仍然存在，这就提示H43Y在中国静脉吸毒者中极有可能起保护作用。
该研究得到了973项目、NSFC-云南联合基金、国家重大科技专项、中国科学院等项目资助和云南省疾病预防控制中心的协助。
附：
郑永唐
郑永唐，1962 年生，研究员，博士、博士生导师。1983 年毕业于江西医学院并获医学学士学位 , 分配至赣南医学院微生物学教研室任助教。1989 年在中国科学院昆明动物研究所获免疫学硕士学位并留所工作。1997 年在中国科学院昆明动物研究所获理学博士学位。 1991 年起历任中国科学院昆明动物研究所助理研究员、副研究员、研究员、博士生导师。1996 年获日本学术振兴会特别资助赴日本北海道大学和群马大学进行 HIV 合作研究。1999 年 -2003 年其间在香港中文大学医学院合作研究一年多。 现为中国科学院昆明动物研究所分子免疫药理学实验室主任，中国科学院昆明生物安全三级实验室 (BSL-3 和 ABSL-3 实验室 ) 主任、 中国科学院西南基地抗病毒天然药物联合实验室副主任。 云南省免疫学会副理事长兼秘书长、云南省细胞生物学会理事、 云南省性病艾滋病防治协会理事、 云南省动 物物学会理事、云南省青年联合会委员、 云南省生物医学伦理审查委员会委员、 《动物学研究》和《中国天然药物》编委。1996 年首批入选中国科学院“西部之光”人才。 1997 年被云南省人民政府授予 “云南省中青年学术和技术学科带头人”称号。 2004 年获云南省有突出贡献的优秀专业技术人才。
研究方向
建立抗 HIV 药物研发的关键技术，充分利用西南动植物丰富的生物资源，重点筛选和研究天然来源的抗HIV 天然化合物和天然药物，对抗 HIV 先导化合物进行构效关系的研究，研发具有我国自主知识产权的抗艾滋病新药；建立 AIDS 灵长类动物模型，进行艾滋病发病机制、药物及疫苗的评价研究，为艾滋病的防治提出新思路和新方法；治疗性基因修饰树状突细胞艾滋病疫苗的研究；建立云南省 HIV 毒种库，开展 HIV 分子流行病学、耐药性、共感染等研究；研究天然免疫分子 TRIM5 在限制 HIV 感染中的作用机制。
主要学术成就
从事免疫学、病毒学和抗 HIV 药物等研究近 20 年，尤其在抗 HIV 药物研究方面积累了丰富的经验，取得了 公认的成绩，研究处于国内领先地位和国际先进水平。 1995 年以来先后主持了 20 多项国家、科学院和云南省的科研项目
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Kunming Institute of Zoology new results published in AIDS Research
www.ebiotrade.com Time: March 2, 2011 0 Source: Biological Link
Abstract: The news from the Kunming Institute of Zoology, Institute of Animal Models and Human Disease Mechanisms, Laboratory researchers in 1294 in Yunnan's Dai Han, or the study of intravenous drug users to obtain information on the genetic polymorphism of TRIM5α HIV-1 susceptibility to the latest developments, research results recently published in academic journals internationally renowned AIDS JAIDS (56:306-311, 2011) on.
Bio-General reported a message from the Kunming Institute of Zoology, Institute of Animal Models and Human Disease Mechanisms, Laboratory researchers in 1294 in Yunnan's Dai Han, or the study of intravenous drug users to obtain the genetic polymorphism on the TRIM5α and HIV-1 susceptibility to the latest developments, research results recently published in academic journals internationally renowned AIDS JAIDS (56:306-311, 2011) on.
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The study is led Kunming Institute of Zoology, ZHENG Yong-tang researchers, ZHENG Yong-tang researchers now Kunming Institute of Zoology, Chinese Academy of Pharmacology Laboratory of Molecular Immunology, selected the first batch of Chinese Academy of Sciences in 1996, "Western Light" talent. People's Government of Yunnan Province in 1997 was awarded the "academic and technical disciplines of Yunnan young leaders," the title. Yunnan Province in 2004 by the outstanding contributions of outstanding technical professionals. Other authors include the Chinese University of Hong Kong Liu Fengliang PhD Professor Deng Liangsheng.
The whole of biological evolution, organisms have evolved a variety of "weapons" against the invasion of viruses. To deal with HIV-1 infection in primate cells for HIV-1 replication processes within a variety of HIV-1 virus replication inhibited the limiting factor, such as the three motif protein 5α (TRIM5α), apolipoprotein B mRNA editing enzyme 3G (APOBEC3G), binding protein (tetherin) and the zinc finger antiviral protein (ZAP) and so on. Often limiting factors and individual genetic polymorphisms and susceptibility to viral infection is closely related to differences in disease progression. Human TRIM5α gene polymorphism and susceptibility to HIV-1 disease progression and the relevance of research to the conclusion not consistent with the scale of China's population and the lack of TRIM5α polymorphism.
In this article, researchers in 1294 in Yunnan Han Dai intravenous drug users or TRIM5α gene polymorphism and susceptibility to HIV-1 related research. A total of 14 genetic variants that, in addition have been reported before the-2G / C, H43Y, V112F, R136Q SNP, and several other important sites, but also the new found I2V, R97C, G110R and other rare mutations . Gene frequency of 4 higher SNP (ie common SNP) analysis of the correlation was found-2G / C, V112F, and R136Q these three SNP genotype frequency and gene frequency, and 4 SNP haplotype in common HIV-1-positive groups and negative groups, no statistically significant difference in the distribution, while the H43Y mutant homozygous genotype (TT) in injection drug users infected with HIV-1 was significantly lower than in the frequency of intravenous drug users not infected with HIV-1 and The statistical difference in terms of the data stratified analysis of national remains, which prompted H43Y in intravenous drug users in China is likely to play a protective role.
The research was supported by 973 Project, NSFC-Yunnan joint fund, major national science and technology projects and other projects funded Chinese Academy of Sciences and Yunnan Provincial Center for Disease Control and Prevention for their assistance.
Attachment:
ZHENG Yong-tang
ZHENG Yong-tang, born in 1962, Professor, Ph.D., Professor. He graduated from Jiangxi Medical College in 1983 and was medical degree, assigned to the Department of Microbiology, Gannan Medical College, Assistant Professor. 1989 Kunming Institute of Zoology Chinese Academy of Sciences received a master's degree and leave the immunological work. 1997 Kunming Institute of Zoology Chinese Academy of Sciences Ph.D.. Since 1991, successive Chinese Academy of Sciences, Kunming Institute of Zoology, Assistant Professor, Associate Research Professor. 1996 by the Japan Society for the Promotion of special funding to go to Japan, Gunma, Hokkaido University and the University of HIV collaborative research. During the years 1999 -2003 at the Chinese University of Hong Kong, more than a year collaborative research. Kunming Institute of Zoology, Chinese Academy of Sciences is currently Pharmacology Laboratory of Molecular Immunology, Kunming Institute of Laboratory biosafety levels (BSL-3 and ABSL-3 lab) Director, Chinese Academy of Sciences Southwest base of natural anti-viral drug combination lab, Officer. Yunnan Immunology vice chairman and secretary general, director of cell biology in Yunnan Province, Yunnan Province Association of STD and AIDS Prevention, Yunnan Province, animal materials Society, Yunnan Youth Federation, Yunnan Province, Biomedical Ethics Review Committee, " Zoology "and" Chinese natural medicine "Editorial Board. Chinese Academy of Sciences in 1996 selected the first batch of "Western Light" talent. People's Government of Yunnan Province in 1997 was awarded the "academic and technical disciplines of Yunnan young leaders," the title. Yunnan Province in 2004 by the outstanding contributions of outstanding technical professionals.
Research
Anti-HIV drug development to establish the key technologies, plants and animals take advantage of the rich biological resources of Southwest, the focus of natural selection and source of natural compounds and natural anti-HIV drugs, lead compounds against HIV structure-activity relationship studies, research and development with our own knowledge property rights, anti-AIDS drugs; established primate model of AIDS, the AIDS pathogenesis, evaluation of drugs and vaccines for the prevention and treatment of AIDS with new ideas and new methods; therapeutic gene-modified dendritic cells in AIDS vaccine research; HIV virus seed bank established in Yunnan Province, to carry out HIV molecular epidemiology, drug resistance, a total of infection studies; of innate immune molecules TRIM5 to restrict HIV infection mechanism.
Main Academic Achievements
In immunology, virology and anti-HIV drugs of the past 20 years, especially in the anti-HIV drug research has accumulated a wealth of experience, has made recognized achievements in research in the leading position in domestic and international advanced level. Since 1995, has presided over more than 20 countries, Academy of Sciences and a research project in Yunnan Province
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